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KMID : 1094720100150030400
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Biotechnology and Bioprocess Engineering
2010 Volume.15 No. 3 p.400 ~ p.406
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Antioxidant Activity of the Chemical Constituents from the Flower Buds of Magnolia denudata
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Seo Young-Wan
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Abstract
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The scavenging activity of the flower buds of Magnolia denudata Desrousseaux on reactive oxygen species (ROS) was evaluated using 2’,7’-dichlorofluorescin diacetate (DCFH-DA) in HT 1080 cells. Methanol (MeOH) and dichloromethane (CH2Cl2) extracts inhibited dosedependently generation of ROS in the cellular system. MeOH and CH2Cl2 extracts were combined and fractionated with n-hexane, 85% aqueous MeOH, and n-butanol (n- BuOH). Both n-hexane-soluble and 85% aqueous-soluble fractions showing strong radical-scavenging activity in the cellular system were further separated by diverse chromatographic methods to give five known lignans (1-5). All these compounds exhibited significant radical-scavenging effect on intracellular ROS in a dose-dependent manner. Their scavenging activity on various reactive oxygen species (ROS) was also evaluated using electron spin resonance (ESR) spin-trap techniques.
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KEYWORD
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Magnolia denudata, antioxidant activity, reactive oxygen species, ESR
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